The liquid MS medium (Murashige Skoog's) was purchase from Sigma-Aldrich, India. Serological pipettes and T75 culture flask were procured from Thermo Scientific, while the culture petridish and 200 μL microtips were purchased from Tarson, USA. All the other chemicals used in this experiment were analytical grade procured from India.

The three plant callus was taken in the experimental group’s viz. plant callus of Mandukparni (Centellaasiatica), Katsarika (Barleriacristata), and Amla (Phyllanthus emblica). For experimental testing, each callus culture and plant MS media were divided into two parts, one is control and other is the Biofield Energy Treated. All the groups were visualized for growth and yield of the callus on day 0 and day 7.

The test samples, three plant callus and MS media were divided into two parts, one part each of the test sample (plant callus and MS media) was treated with the Biofield Energy by a renowned Biofield Energy Healer (also known as The Trivedi Effect^®) and coded as the Biofield Energy Treated DMEM, while the second part did not receive any sort of treatment in the control test sample (plant callus and MS media) group. This Biofield Energy Healing Treatment was provided by Gopal Nayak remotely for ~5 minutes, while the test item was located in the research laboratory of Dabur Research Foundation, New Delhi, India. This Biofield Energy Treatment was administered for 5 minutes through the Healer’s unique Energy Transmission process remotely to the test sample under laboratory conditions. Gopal Nayak in this study never visited the laboratory in person, nor had any contact with the test sample (plant callus and MS media). Further, the control groups were treated with a sham healer for comparative purposes. The sham healer did not have any knowledge about the Biofield Energy Treatment. After that, the Biofield Energy Treated and untreated samples were kept in similar sealed conditions for experimental study. After that, the Biofield Energy Treated and untreated samples were kept in similar sealed conditions for experimental study.

Healthy leaflet of the mandukparni, katsarika, and amla explants were collected in appropriately sized. All the three explants were thoroughly washed using running tap water for 20 minutes in order to remove the traces of dust, foreign materials, etc. followed by washing with the fungicide solution. Further, the explants were exposed to surface sterilization using 70% ethanol for about 40 seconds and again washed 4-5 times using sterile double distilled water.

All the three fine cuts were made in the young leaf (wound induction), which were cultured on MS basal medium, gelled with 0.8% agar containing 3% sucrose. The pH of the callus medium was adjusted approximately 5.8 before agar was gelled. All the plant callus cultures were maintained in growth condition at 25 ± 2º C and 60% to 70% relative humidity with a photoperiod of 16 hour at 3000 lux light intensity with a photoperiod of 16 hour light and 8 hour dark.

All the three cultures were incubated in a culture room and after some days, callus induction was started in all the samples. Further, the suspension culture was made from the friable creamish calli, which was transferred to the T75 culture flasks in liquid MS media supplemented with different phytohormones such as cytokines (6-benzylaminopurine, BAP with 5.0 mg/L and kinetin, KIN with 0.5 mg/L concentration) and auxins (α-naphthalene acetic acid, NAA with 2.0 mg/L and 2,4-dichlorophenoxyacetic acid, 2,4-Dwith 2.0 mg/L). The aliquots of mandukparni, katsarika, and amla stem cells were cultured in T-75 cell culture flasks. The flasks received Biofield Energy Treatment (known as The Trivedi Effect^®) at this stage without touching the cells. Following the treatment, the above T-75 flasks were incubated till day 7 in a shaking incubator (120 rpm) under photoperiod of 16 hours at 3000 lux light intensity provided by cool white fluorescent tubes. The incubation conditions were similar to that of untreated cells. On day 7, the flasks were observed and visualized under phase contrast microscope for monitoring cell growth and photomicrographs were captured at 200X magnification. The wet weight of all the callus was taken after day 7 39.

Mandukparni (Centellaasiatica) growth and yield of plant callus after Biofield Energy Healing Treatment and the results are presented in Figure 1, while the percentage increase in the callus weight was presented in Figure 4. Mandukparni callus flasks were observed and visualized under phase contrast microscope for cell growth and photomicrographs were captured at 200X magnification on day 0 and day 7 in both the groups, which was compared. The plant callus at day 7 as presented showed significant growth after Biofield Energy Treatment, while the callus growth was high on day 7 as compared with the untreated plant callus and MS media. However, experimental data suggested that the Mandukparni weight of callus on day 0 in the untreated group was 323 mg, while it was 300 mg in the Biofield Energy Treated group. On the other hand, on day 7, the untreated and Biofield Energy Treated callus weight was 620 and 630 mg, respectively. The weight increase in untreated group was 297 mg, while Biofield Energy Treated group showed a weight increase of 330 mg. Overall, the data showed 11.1% increased in the plant callus growth on day 7 after Biofield Energy Healing Treatment as compared with the control group. The experimental data suggested the significant callus growth, which have been used for many biological activities such as antioxidant, anti-feedant, anti-leprotic, antibacterial, and anti-tuberculosis activity along with significant use in leprosy and tuberculosis due to the presence of brahminoside, asiaticoside, indocentelloside, brahmoside, and theankuniside 404142. Biofield Energy Treatment showed an improved callus growth of Centellaasiatica, which can be used against many biological disorders. Overall, the experimental data assumed that The Trivedi Effect^® has significant capacity to improve the callus growth that can be used for isolation of biological active constituents, which have wide application against various pharmacological activities.

Katsarika plant callus growth and yield after Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Gopal Nayak showed a significant growth and the results are presented in Figure 2. Katsarika callus flasks were observed and visualized under phase contrast microscope for cell growth and photomicrographs were captured at 200X magnification on day 0 and day 7. The plant callus at day 7 as presented in the Figure 2, showed a significant growth after Biofield Energy Treatment, while the callus growth was high on day 7 as compared with the untreated plant callus and MS media. The experimental data revealed that the katsarika weight of callus (day 0) in the untreated group was 290 mg, while it was 302 mg in the Biofield Energy Treated group. On the other hand, on day 7 the katsarika weight of callus in the untreated group was 605 mg, while it was 782 mg in the Biofield Energy Treated group. The weight increase in control group was 315 mg, while Biofield Energy Treated group showed a weight increase of 480 mg. Overall, the data showed 52.4%increased in plant callus growth on day 7 as compared with the untreated control (day 0). The results suggested that Biofield Energy Healing Treatment has significant capacity to improve the plant callus of Barleriacristata. Thus, it can be used against isolation of vital phytoconstituents like iridoids, flavonoids, and phenylethanoid derivatives 10.

The results of Amla plant callus growth and yield after Biofield Energy Healing Treatment are presented in Figure 3. Amla callus flasks were observed and visualized under phase contrast microscope for cell growth and photomicrographs were captured at 200X magnification on day 0 and day 7. The plant callus at day 7 showed a significant improved callus growth after Biofield Energy Treatment as compared with the untreated plant callus. However, results suggested that the amla plant callus weight in the untreated group was 403 mg, while it was 369 mg in the Biofield Energy Treated group on day 0. On the other hand, on day 7 the amla callus weight in the untreated group was 730 mg, while it was 920 mg in the Biofield Energy Treated group. The weight increase in control group was 327 mg, while Biofield Energy Treated group showed a weight increase of 551 mg. Overall, the data showed 68.5% increase in amla plant callus growth as compared with the untreated control. Phyllanthus emblica is one of the best dietary sources of vitamin C, amino acids, and minerals. It contains emblicol, phyllembelic acid, phyllembelin, rutin, curcuminoids, phenolic compounds and tannins as the major constituents for various biological activities. Biofield Energy Healing Treatment showed a significant improved growth of plant callus, which can be highly useful to isolate the important phytoconstituents. Amla has been reported with significant biological activities such as antidiarrheal, antitussive, analgesic, hepatoprotective, wound healing, anti-atherogenic, anti-atherosclerotic, adaptogenic, gastro-protective, anti-hypercholesterolemia, nephron-protective, and neuroprotective properties 434445.

Overall, the comparative data of all the three plants after treatment with The Trivedi Effect^®-Energy of Consciousness Healing Treatment showed a significant increased in plant callus weight of Mandukparni (Centellaasiatica), Katsarika (Barleriacristata) and Amla (Phyllanthus emblica). However, Centellaasiatica, Barleriacristata, and Phyllanthus emblica showed a significant improved callus weight by 11.1%, 52.4%, and 68.5%, respectively on day 7 as compared with the day 0 (Figure 4).

Authors are grateful to Dabur Research Foundation, Trivedi Science, Trivedi Global, Inc., Trivedi Testimonials and Trivedi Master Wellness for their support throughout the work.

MS: Murashige and Skoog (1962) medium

NAA: α-naphthalene acetic acid

IAA: Indole-3-acetic acid

2,4-D: 2,4-dichlorophenoxyacetic acid

BAP: 6-benzylaminopurine

KIN: Kinetin

NPs: Natural Products