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May 2020 DOI 10.14302/issn.2641-4538.jphi-20-3329
A.J ThatheyusCorresponding author
PG & Research Department of Zoology, The American College, Madurai, Tamil Nadu, India.
Pesticides are applied in agricultural fields for controlling pest population to achieve crop protection. But they cause damage to nontarget organisms and affect the quality of environment including water, air and soil. The present study has been designed to test the efficiency of Pseudomonas stutzerion the degradation of malathion. The bacterial strain was subjected to 50, 100, 150 and 200 ppm of malathion in minimal broth for 30 hours and changes in orthophosphate levels, pH and turbidity were monitored for every six hours. Efficiency of free and immobilized cells were compared for orthophosphate release. Influence of different sugars on degradation was also compared. Degradation of 150 ppm of malathion was confirmed with UV-Visible spectrophotometric analysis and HPLC analysis. The data were subjected to two way analysis of variance and the results are discussed. (Graph 1) Graph 1. Graphical Abstract
Apr 2020 DOI 10.14302/issn.2576-6694.jbbs-20-3282
A.J ThatheyusCorresponding author
PG & Research Department of Zoology, The American College, Madurai, Tamil Nadu, India.
Pesticides applied in agricultural fields for crop protection result in the contamination of the environment. They also affect the flora and fauna as well as the quality of air, water and soil. Hence their remediation is of concern. Among different strategies available, microbial remediation is cost effective and ecofriendly. The present work is designed to test the efficiency of Pseudomonas stutzeri, a bacterial strain obtained from MTCC, IMTECH, Chandigarh, India. After treating with 50, 100, 150 and 200 ppm of methyl parathion, parameters like, orthophosphate released, pH and turbidity changes were monitored upto thirty hours. Degradation of 200 ppm concentration was confirmed by UV-Visible spectrophotometry and HPLC analysis. This strain effectively degraded methyl parathion and it can be used in the remediation of contaminated agricultural fields.
Oct 2023 DOI 10.14302/issn.2769-2264.jw-23-4694
Alotaibi MajebCorresponding author
This study aims to determine an empirical relationship between the biological and chemical oxygen demands of industrial effluent running through the membrane bio reactor-based Al-Hasa common industrial wastewater treatment Plant. The relationship between these two parameters was investigated over one month (NOV– 2021 to OCT-2022). The values of R² show that its impossible to predict the result of BOD5 based on COD, however the result of r in AUG month shows a strong relation between them. The average biodegradability index over the one year is less than 0.6, showing that effluent is highly polluted, and biodegradation will not proceed. The plant’s performance in terms of BOD5 and COD removal was very poor over the whole year, however, the months AUG, SEP and OCT months shows a satisfactory performance, this is because a large amount of Industrial wastewater entering the plant contains both organic and inorganic components.
Jun 2023
Rifkatu Kambel DogaraCorresponding author
The increasing demand for environmentally-friendly materials has led to a surge in research on the production of biodegradable polymers. In this study, we investigate the synthesis of a biodegradable polymer by graft copolymerization of gum Arabic (GA) and polyethylene glycol (PEG). GA, a natural polysaccharide and PEG, a synthetic water-soluble polymer, were used as the backbone and graft monomer, respectively. The graft copolymerization was carried out using benzoyl peroxide as an initiator and performed under nitrogen atmosphere. The resulting polymer was characterized by Fourier transform infrared (FTIR) spectroscopy, Xray diffraction (XRD), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). The FTIR spectra confirmed the formation of the graft copolymer, and TGA analysis showed that the copolymer had higher thermal stability than GA. The DTA thermograms indicated two thermal events. The evaporation of water and organic polyethylene glycol components was measured, and the first mass loss was due to the loss of adsorbed and structural water in the gum Arabic, which occurred between 31.87 and 180°C, while the second, corresponding to the pyrolysis of polyethylene glycol functional groups and polysaccharide decomposition, resulted in a 70% mass loss. SEM morphological analysis of gum Arabic showed aggregates of high irregularity in particle shape. The cracks and holes obtained in the Gum Arabic micrograph disappeared from the new gum Arabic-graft-polyethylene glycol, leaving a smooth surface with scattered particles in the image, which was due to the grafting copolymer. From the XRD patterns, the percentages of the amorphous and crystalline phases were determined. The results show that gum Arabic has a 78% degree of crystallinity, whereas gum Arabic-graft-polyethylene glycol has the lowest value of 51%. Biodegradation activity was observed using the fungus Aspergillus flavus on different days on gum Arabic-g-polyethylene glycol. The results clearly showed inhibition zones with a change in the state of the copolymer from solid to liquid from days 8 to 14. These results indicate that the GA-PEG copolymer has potential as a biodegradable material for use in various applications, such as packaging, agriculture, and medical industries.
Jul 2020 DOI 10.14302/issn.2377-2549.jndc-20-3460
Tamilvanan NivedharshiniCorresponding author
India
Laccase enzyme production is important and more beneficial for environment, because it has many roles like, involved in bioremediation, biodegradation, decolorization of environmental polluted dyes and pharmaceutical sector also. Production of laacse enzyme from bacillus sp as using of Agro waste (rice bran) as a substrate. The Agricultural soil sample was collected, after the sample were processed for the preliminary and biochemical tests to identification of Bacillus organism. The Guiacol inducer were used for microbial screening of laccase enzyme production. After that microbial screening, various optimization parameters (pH, Temperature, Inducers, carbon and nitrogen sources) are checked for that production of laccase enzyme in mass level. Based on that optimization the bulk fermentation (large scale) (solid state fermentation) were done as a rice bran substrate. The fermentation product was subjected to analyzed the physiochemical properties and purification based on that techniques of Gel filtration chromatography, Dialysis, Ammonium sulfate preciptation. The protein estimation of that product to analysed by lowry’s method.