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Jan 2021 DOI 10.14302/issn.2639-3166.jar-20-3677
Faruk IqbalCorresponding author
Principal Scientific Officer, Plant Pathology Division, BARI
The experiments were conducted in the fields of Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur during 2016-17, 2017-18 and 2018-19 cropping years to evaluate the organic and vermi composts for mass culturing of biological control agent Trichoderma harzianum and toobserve the effect of formulated T.harzianum designated as Tricho-vermi-compost and Tricho-organic-compost as well as organic compost, vermi-compost and chemical fungicide Provax 200 WP against soil-borne pathogens, Sclerotium rolfsiiof groundnut causing foot and root rot/stem rot disease. The pathogen inoculated field soils were treated with Tricho-vermi-compost and Tricho-organic-compost, organic compost and vermi-compost 7 days before seed sowing where as seeds were treated with Provax 200 WP at the time of seed sowing. From this study it was revealed that all the treatments performed in reducing seedling mortality and increasing plant growth and yield of groundnut compared to control. Among the treatments, soil treatment with Tricho-vermi-compost and Tricho-organic-compost are the best treatments in reducing seedling mortality and increasing plant growth parameters and yield of groundnut which was significantly differed from the other treatments including control. Seed treatment with chemical fungicide Provax 200 WP and soil treatment with only vermin-compost and organic compost also promising treatments for management foot and root rot disease and increasing plant growth parameters as well as yield of groundnut compared to control.
Dec 2019 DOI 10.14302/issn.2690-4829.jen-19-3105
Brumm PhillipCorresponding author
C5-6 Technologies LLC, 5627 Old Oak Drive, Fitchburg, WI 53711, USA
Trichoderma reeseiβ-glucosidase (Bgl1) is one of four enzymes demonstrated to act synergistically to degrade cellulose both in vitro and in vivo. Our work attempted to better understand the substrate specificity and potential biotechnological applications of Bgl1. T. reesei Bgl1H cleaves over 80% of the β-(1-4) and β-(1-3) linkages in β-glucan and 14% of the β-(1-4) linkages in amorphous cellulose, significantly more than any tested bacterial β-glucosidase. Bgl1H cleaves 50% of the β-(1-4) linkages in xyloglucan when supplemented with cellulase and α-xyloside. Approximately 20% conversion to glucose was obtained from insoluble β-(1,3)-linked curdlan using only Bgl1H; addition of a curdlanase resulted in conversion of approximately 70% of the curdlan to glucose. Bgl1H also produces xylose from xylooligosaccharides and debranched xylans. For both glucans and xylans, the relative rates of hydrolysis increase with increasing polysaccharide chain lengths. Bgl1H is able to partially degrade β-glucan in a variety of grain components; addition of endo-acting enzymes improved the enzyme’s performance on these grain components. The ability of this enzyme to produce monosaccharides from undigestible polysaccharides suggest it may have potential in improving utilization of carbohydrates in animal feed, fermentations, and other biotechnological applications.
Jun 2022 DOI 10.14302/issn.2832-5311.jpcd-22-4182
Md. Iqbal FarukCorresponding author
Sclerotiniasclerotiorum, the causal agent for white mold (Sclerotinia stem rot), is a devastating fungal pathogen. Currently, Sclerotinia is most commonly managed using the chemical fungicide which can lead to Sclerotinia resistance development, impacting biodiversity and interfering with key ecosystem services. In this regards, field experiments were conducted during 2017-18 planting seasons to evaluate the efficacy of different components viz. sawdust burning, stable bleaching powder, fungal and bacterial bio-control agents, chemical fungicide Rovral 50 WP and integration of different components for the management white mold disease of bush bean, mustard and garden pea in three different locations viz. in the field of Plant Pathology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Regional Agricultural Research Station (RARS), Burirhat, Rangpur and RARS, Ishurdi, Pabna, respectively. The results showed that different treatments displayed varying levels of effectiveness against the disease. All the treatments gave satisfactory reduction of white mold disease development and increased plant growth as well as yield of bush bean, mustard and garden pea. Among the treatments, integration of saw dust burning + soil amendments with Trichoderma based bio-fungicide + bacillus based bio-control agents + application fungicide Rovral 50 WP is the best treatment which reduced 97.49%, 77.72%, 72.26% white mold disease incidence and 84.61%, 81.14%, 71.01% white mold disease severity of mustard, bush bean and garden pea, respectively and increasing plant growth parameter as well as 52.16%, 27.74%, 36.97% yield of mustard, bush bean and garden pea, respectively. Application of only fungicide Rovral 50 WP also better treatment in reduction of white mold disease incidence and disease severity and increasing plant growth parameter as well as increasing yield of mustard, bush bean and garden pea. Soil amendment with fungal or bacterial bio-control agents also gave satisfactory results in reduction of white mold disease incidence and disease severity and increasing plant growth parameter as well as increasing yield of mustard, bush bean and garden pea. It could be concluded from the obtained results that integration between bio-control agents as a soil treatment and foliar application chemical fungicide might be useful as a good tool for controlling white mold disease caused by S.sclerotiorum and obtained higher yield of bush bean, mustard and garden pea under field condition.
Feb 2022 DOI 10.14302/issn.2639-3166.jar-21-4019
Faruk IqbalCorresponding author
Principal Scientific Officer, Plant Pathology Division, Agricultural Research Institute, Joydebpur, Bangladesh.
This article has been retracted on 10 November 2022. VIEW THE RETRACTION NOTICE (https://doi.org/10.14302/issn.2639-3166.jar-25-5841) A field experiment was conducted with a view to test the efficacy of Tricho-composts and integration of Tricho-composts, poultry refuse (PR), neem oilcake (NOC), saw dust burning (SDB) with chemical nematicide Furadan 5G (Carbofuran) for controlling root knot nematode of country bean through which growers will be benefited. The root-knot nematode infested soils were treated with Tricho-compost-1 @ 2 kg/pit, Tricho-compost-2 @ 2 kg/pit, Tricho-compost-1 @ 2 kg/pit + Furadan 5 G @ 20 g/pit, Tricho-compost-2 @ 2 kg/pit + Furadan 5 G @ 20 g/pit, poultry refuse @ 5-6 kg/pit + Furadan 5 G @20 g/pit , neem oil cake @ 500 g/pit + Furadan 5 G @ 20 g/pit and saw dust burning + Furadan 5 G @ 20 g/pit. It was revealed that all the treatments performed well in reducing root-knot nematode infestation and increasing plant growth and yield country bean compared to farmers’ practices. Among the treatments, integration of poultry refuse with Furadan 5G or Trichoderma based bio-fungicides Tricho-composts with Furadan 5G or neem oil cake with Furadan 5G are the best treatments in reducing root-knot nematode infestation and increasing plant growth as well as yield of country bean. Soil treatment with only Tricho-composts or integration of saw dust burning with Furadan 5G also performed better in reduction of root knot nematode disease and increasing plant growth as well as yield of country bean.